Detecting and Genotyping Respiratory Syncytial Virus and Parainfluenza Viruses: A Cross-Sectional Study in the Etiology of Pneumonia Among Hospitalized Patients in Sarawak, Malaysia
May 15, 2017 - September 30, 2017
Worldwide, lower respiratory tract infections have remained among the top three leading causes of the global burden of disease since 1990 and the leading cause of death among children under five. Sibu Hospital in Sarawak, Malaysia has seen increasing pneumonia admissions with 1,903 admissions recorded in 2015. Kapit Hospital in Sarawak also sees a relatively large number of pneumonia admissions, approximately 300 annually. In this cross-sectional, 10-week study I will determine the etiology of approximately 100 pneumonia admissions at Sibu and Kapit Hospitals. The study will examine demographic, viral, behavioral and occupational risk factors for these admissions, to include severe versus routine pneumonia. Specifically, enrolled subjects’ nasopharyngeal swabs will be examined and genetically characterized with molecular assays for respiratory syncytial viruses (subtypes A and B) and parainfluenza viruses (PIV 1,2,3 &4). I will assess viral transmission in the air in the hospital rooms of patients under 5 years of age. Study subjects will be enrolled at Sibu or Kapit Hospital using an informed consent process (assent for children 7-18 years) that permits the completion of a brief questionnaire, collection of an NP swab, and the deployment of a bioaerosol sampler. The short enrollment questionnaire will capture baseline demographic information, health conditions, and history of animal exposures. Patients will be assigned a study number and personal identifying data will be minimized. Should assays suggest a specimen may harbor a novel microbe, part of that specimen may be shared with laboratories at Duke University, Duke-NUS or other collaborating institutions for further study.