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Faculty

Nimmi Ramanujam

Professor, Biomedical Engineering and Global Health
Pratt School of Engineering
Biomedical Engineering

2575 FCIEMAS
(919) 660-5307
nimmi.ramanujam@duke.edu

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Nimmi Ramanujam

Summary

Since coming to Duke in 2005, Ramanujam has established a lab (http://nimmi.bme.duke.edu/) that is developing and applying innovative optical strategies and technologies for cancer screening in resource-limited settings, detecting residual disease during cancer surgery, and visualizing tumor hypoxia and metabolism in the context of cancer therapy and drug discovery. She is currently working with DGHI partners at the Family Health Ministries to develop a portable, low-power spectroscopic device for screening cervical cancer in Haiti.

Projects

Recent Publications

Frees AF, McCachren III SS, Lee M, Murphy HA, Rajaram N, Dewhirst MW, and Ramanujam N. "Hyperspectral Imaging of Glucose Uptake, Mitochondrial Membrane Potential, and Vascular Oxygenation Differentiates Breast Cancers with Distinct Metastatic Potential In Vivo." Oral presentation. CTh4A.6. Cancer Imaging and Therapy Conference, OSA Biomedical Optics Congress, Hollywood, Florida, April 2016.

Hu F, Liu H, Everitt J, Farsiu S, Ramanujam N, “Label-free vascular imaging in a spontaneous hamster cheek pouch carcinogen model for pre-cancer detection”, SPIE Photonics West, San Francisco, CA, February 2016

Asiedu MN, Agudogo J, Schmitt JW, Miros R, Preschold-Bell, RJ, Krieger M, Ramanujam N. Design and preliminary analysis of a vaginal inserter for speculum-free self-cervical cancer screening. PLOS ONE, 2016. In preparation.

Lam C, Mueller J, Asma B, Asiedu M, Krieger M, Chitalia R, Dahl D, Schmitt J, Ramanujam N. Integrated Strategy for Improving Durability, Contrast, and Portability of the POCkeT Colposcope for Cervical Cancer Screening. Optics Express, 2016. In preparation.

Morhard R, Hu F, Barero Castedo C, Madonna M, Katz D, Ramanujam N. Optimization of ethanol ablation for non-capsulated carcinomas — a potential solution for non-surgical interventions in low resource settings. Journal or Drug Delivery, 2016. In preparation.

Martinez, A, McCachren III S, Lee M, Murphy H, Ashcraft k, Dewhirst M, Ramanujam N. High resolution imaging of glucose uptake, mitochondrial membrane potential, and vascular oxygenation enables phenotyping of heterogeneous murine mammary tumors in living mice. Cancer Research, 2016. In preparation

Crouch B, Murphy H, Belonwu S, Martinez A, Lee M, Hughes P, Haystead T, Ramanujam N. Fluorescently-tethered Hsp90 inhibitors provide diagnostic and prognostic information in breast cancer pre-clinical models. Cancer Research, 2016. In preparation

Hu F, Morhard R, Zhu C, Everitt J, Dewhirst M, Ramanujam N “Label-free vascular imaging in an inducible hamster cheek pouch carcinogen model for pre-cancer detection”, Cancer Research, 2016, in preparation.

Mueller J, Chitalia R, Whitley M, Fu H, Cardona D, Willett R, Kirsch D, Ramanujam N. Comparison between acridine orange and tetracycline staining of preclinical mouse sarcoma. JBO Letters, 2016. Submitted.

Mueller J, Hussain N, Cheatham A, Peters J, Proeschold Bell RJ, Ramanujam N. Integrating human centered design and global service learning to increase retention of women in engineering. Annals of Biomedical Engineering, 2016. In preparation.

Asma B, Mueller J, Lam C, Krieger M, Asiedu M, Gallagher J, Hariprasad R, Malliga J, Mchome B, Oneko O, Taylor P, Venegas G, Wanyoro A, Muasher L, Schmitt J, Ramanujam N. Evaluation of a point of care tampon (POCkeT) colposcope as a screening device for cervical cancer among international physicians. PLOS ONE, 2016. Submitted

Hu F, Morhard R, Murphy H, Zhu C, Ramanujam N, “Dark field optical imaging reveals vascular changes in a spontaneous hamster cheek pouch model during carcinogenesis”, Biomedical Optics Express, 2016. Accepted.

Hu F, Vishwanath K, Salama JK, Erkanli A, Peterson B, Oleson JR, Lee WT, Brizel DM, Ramanujam N, Dewhirst MW, Oxygen and perfusion kinetics in response to fractionatedradiotherapy in FaDu head and neck cancer xenografts are related to treatment outcome, International Journal of Radiation Oncology • Biology • Physics (2016). Accepted.

Chitalia R, Mueller J, Fu H, Whitley M, Kirsch D, Willett R, Ramanujam N, "Comparison of techniques for the segmentation of fluorescent positive features across microscopy systems", Biomedical Optics Express, 2016. Accepted.

Kennedy S, Caldwell M, Bydlon T, Mulvey C, Mueller J, Wilke L, Barry W, Ramanujam N, Geradts J. Correlation of breast tissue histology and optical signatures to improve margin assessment techniques. JBO, 2016. Accepted.

Mueller J, Gallagher J, Chitalia R, Krieger M, Erkanli A, Willett R, Geradts J, Ramanujam N, "Rapid staining and imaging of sub-nuclear features to differentiate between malignant and benign breast tissues at a point-of-care setting", Journal of Cancer Research and Clinical Oncology, 2016 Apr 22. [Epub ahead of print].

Fu HL, Mueller JL, Whitley MJ, Cardona DM, Kirsch DG, Brown JQ, Ramanujam N, “Structured illumination microscopy and a quantitative image analysis for the detection of positive margins in a pre-clinical genetically engineered mouse model of sarcoma”, PLoS one, 2016, 11(1): e0147006.

Lazarides A, Whitley M, Strasfeld D, Cardona D, Ferrer J, Mueller J, Fu H, Bartholf DeWitt S, Brigman B, Ramanujam N, Kirsch D, Eward W, "A fluorescence-guided laser ablation system for removal of residual cancer in a mouse model of soft tissue sarcoma", Theranostics, 2016, 6(2): 155-166

Chitalia R, Mueller J, Fu H, Whitley M, Kirsch D, Willett R, Ramanujam N, "Comparison of techniques for the segmentation of fluorescent positive features across microscopy systems", Biomedical Optics Express, 2015 in preparation.

Mueller J, Chitalia R, Whitley M, Fu H, Cardona D, Willett R, Kirsch D, Ramanujam N, "Comparison between acridine orange and tetracycline staining of preclinical mouse sarcoma", JBO Letters, 2015 in preparation.

Mueller J, Gallagher J, Chitalia R, Krieger M, Erkanli A, Willett R, Geradts J, Ramanujam N, "Rapid staining and imaging of sub-nuclear features to differentiate between malignant and benign breast tissues at a point-of-care setting", Journal of Cancer Research and Clinical Oncology, 2015 submitted.

Mueller J, Gallagher J, Erkanli A, Geradts J, Ramanujam N, "Rapid staining and imaging of sub-nuclear features for differentiating between malignant from benign breast tissues", PLoS ONE, 2015 submitted.

Fu HL, Mueller JL, Whitley MJ, Cardona DM, Kirsch DG, Brown JQ, Ramanujam N, "Structured illumination microscopy and a quantitative image analysis for the detection of positive margins in pre-clinical genetically engineered mouse model of sarcoma," PLOS ONE, 2015 accepted.

Lazarides A, Whitley M, Strasfeld D, Cardona D, Ferrer J, Mueller J, Fu H, Bartholf DeWitt S, Brigman B, Ramanujam N, Kirsch D, Eward W, "A fluorescence-guided laser ablation system for removal of residual cancer in a mouse model of soft tissue sarcoma", Theranostics, 2015, 6(2): 155-166, in press.

Lam CT, Krieger MS, Gallagher JE, Asma B, Muasher LC, Schmitt JW, Ramanujam N, "Design of a Novel Low Cost Point of Care Tampon (POCkeT) Colposcope for Use in Resource Limited Settings", PloS ONE, 10(9), doi: 10.1371/journal.pone.0135869, September 2015.

Dobbs J, Mueller J, Krishnamurthy S, Shin D, Kuerer H, Yang W, Ramanujam N, Richards-Kortum R. "Micro-anatomical quantitative optical imaging: towards automated assessment of breast tissues", Breast Cancer Research, 17: 105. doi: 10.1186/s13058-015-0617-9, August 2015, 17:105.

Crouch BT, Murphy HA, Haystead T, Ramanujam N, "A Novel 'See-and-Treat' Paradigm for Breast Cancer Using Fluorescently-Tethered Hsp90 Inhibitors", ECI (15AO) Advances in Optics for Biotechnology, Medicine, and Surgery XIV, Vail CO, June 14-17 2015.

Frees AE, McCachren SS, Rajaram N, Dewhirst MW, Ramanujam N, "Optical toolbox for in vivo analysis of glucose uptake, vascular oxygenation, and mitochondrial membrane potential in breast cancer", AACR Special Conference: Metabolism and Cancer, Bellevue, WA, June 7-10 2015.

Nichols BS, Schindler CE, Brown JQ, Wilke LG, Mulvey CS, Krieger MS, Gallagher J, Geradts J, Greenup R, von Windheim K, Ramanujam N, "A Quantitative Diffuse Reflectance Imaging (QDRI) System for Comprehensive Surveillance of the Morphological Landscape in Breast Tumor Margins", PloS ONE, 10, e0127525, June 2015.

Mueller J, Fu H, Mito J, Whitley M, Chitalia R, Erkanli A, Dodd L, Cardona D, Geradts J, Willett R, Kirsch D, Ramanujam N, "A quantitative microscopic approach to predict local recurrence based on in vivo intraoperative imaging of sarcoma tumor margins", International Journal of Cancer, 137(10): 2403-12. doi: 10.1002/ijc.29611, June 2015.

Lam CT, Ramanujam N, "Design and Quantitative Evaluation of a Novel Low Cost Trans-Vaginal Digital Colposcope for use in Resource-Limited Settings", Oral Presentation at Unite for Sight Global Health and Innovation Conference 2015, in New Haven, CT, March 29, 2015.

Bydlon TM, Nachabé R, Ramanujam N, Sterenborg HJCM, Hendriks BHW, "Chromophore based analyses of steady-state diffuse reflectance spectroscopy: current status and perspectives for clinical adoption", Journal of Biophotonics, 8, No. 1-2, 9-24 (2015) / DOI 10.1002/jbio.201300198, January 2015.

Rajaram N, Reesor A, Mulvey C, Frees AE, Ramanujam N, "Non-invasive, simultaneous quantification of vascular oxygenation and glucose uptake in tissue", PLoS ONE 10(1): e0117132, doi:10.1371/journal.pone.0117132, January 2015.

Hu F, Vishwanath K, Wolfgang Beumer H, Puscas L, Afshari HR, Esclamado RM, Scher R, Fisher S, Lo J, Mulvey C, Ramanujam N, Lee WT. "Assessment of the sensitivity and specificity of tissue-specific-based and anatomical-based optical biomarkers for rapid detection of human head and neck squamous cell carcinoma". Oral oncology 50(9):848-856 Sep 2014. DOI: 10.1016/j.oraloncology.2014.06.015.

Nichols BS, Llopis A, Senlik, O Miller, D Schindler CE, Palmer G, Brroke MA, Jokerst NM, Ramanujam N, "A Portable Spectral Imaging System for Wide-field, Sub-millimeter Imaging of the Morphological Landscape of Breast Tumor Margins", OPTICS LETTERS, 2014 submitted.

Yu B, Shah A, Wang B, Rajaram N, Wang Q, Ramanujam N, Palmer GM, Dewhirst MW, "Measuring tumor cycling hypoxia and angiogenesis using a side-firing fiber optic probe," J Biophotonics 7(7):552-564 Jul 2014.

Frees, AE, Rajaram N, McCachren III, SS, Fontanella AN, Dewhirst MW, and Ramanujam N, "Delivery-corrected imaging of fluorescently-labeled glucose reveals distinct breast cancer phenotypes," PLOS ONE, Submitted, October 2014.

Rajaram, N, Reesor A, Mulvey C, Frees AE, and Ramanujam N, "Non-invasive, simultaneous quantification of vascular oxygenation and glucose uptake in tissue", PLOS ONE, Submitted, September 2014.

J. Quincy Brown and Torre M. Bydlon, Stephanie A. Kennedy, Matthew L. Caldwell, Jennifer E. Gallagher, Marlee Junker, Lee G. Wilke, William T. Barry, Joseph Geradts, Nimmi Ramanujam. "Leveraging Tissue Composition and Micromorphology for Breast Tumor Margin Assessment via Quantitative Diffuse Optical Spectral Imaging." (in progress)

Barrott JJ, Hughes PF, Osada T, Yang XY, Hartman ZC, Loiselle DR, Spector N, Neckers L, Rajaram N, Hu F, Ramanujam N, Vaidyanathan G, Affleck DJ, Zalutsky MR, Lyerly HK and Haystead T, "Optical and radioiodinated tethered Hsp90 inhibitors reveal selective internalization of ectopic Hsp90 in malignant breast tumor cells," Chemistry and Biology, September 2013.

Hu F, Vishwanath K, Lo JY, Lee WT, Ramanujam N, Rapid determination of oxygen saturation and vascularity for oncological applications, (in progress).

Hu F, Visshwanath K, Beumer, W.H., Puscas L, Afshari H, Esclasmado R, Scher R. Fisher S, Ramanujam N, Lee W, "Tissue-Specific-based Assessment of Physiological and Morphological Biomarkers Enables Rapid Detection of Human Head and Neck Squamous Cell Carcinoma," Oral Oncology, Submitted October 2013.

Vishwanath K, Palmer GM, Brown JQ, Ramanujam N, "Non-invasive and quantitative sensing of tumor physiology and function via steady-state diffuse optical spectroscopy " in Biosensors and biodetection technologies for cancer detection, diagnostics and research, Rasooly, A. and Herold, K., ed. (CRC Press).

Yu B, Shah A, Wang B, Rajaram N, Wang Q, Ramanujam N, Palmer GM, Dewhirst MW, "Measuring tumor cycling hypoxia and angiogenesis using a side-firing fiber optic probe. Journal of Biophotonics (published online) Dec 2012.

Liu C, Rajaram N, Vishwanath K, Jiang T, Palmer G, Ramanujam N. Experimental Validation of an Inverse Fluorescence Monte Carlo Model to Extract Concentrations of Metabolically Relevant Fluorophores from Turbid Phantoms and a Murine Tumor Model. Journal of Biomedical Optics 17(7):077012, 2012

Lo JY, Brown JQ, Dhar S, Yu B, Jokerst NM, Ramanujam N. Wavelength optimization for quantitative spectral imaging of breast tumor margins, PLoS ONE, Submitted 2012.

Dhar S, Lo JY, Palmer GM, Brooke MA, Nichols BS, Yu B, Ramanujam N, Jokerst NM, "A diffuse reflectance spectral imaging system for tumor margin assessment using custom annular photodiode arrays," Biomedical Optics Express, December 2012

Mueller J, Harmany Z, Mito K, Kennedy S, Kim Y, Dodd L, Geradts J, Kirsch D, Willett R, Brown Q, Ramanujam N. Quantitative segmentation of fluorescence microscopy images of heterogeneous tissue: Application to the detection of residual disease in tumor margins. PLoS one, 2012.

Chang VTC, Merisier D, Yu B, Walmer DK, and Ramanujam N, "Towards a field-compatible optical Spectroscopic device for cervical cancer screening in resource-limited settings: effects of calibration and pressure," Optics Express, 19(19), 17908-17924, September 2011.

Brown JQ, Vishwanath K, Chang TVC, Ramanujam N, "Clinical Applications of UV-VIS Optical Spectroscopy in Cervix, Head and Neck, and Breast Cancer," in In Vivo Clinical Imaging and Diagnosis, J. Tunnell, ed. 239-285, 2011.