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Affiliate Faculty

Guido Ferrari

Assistant Professor, Surgery
School of Medicine

(919) 684-2862

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Guido Ferrari


Dr. Ferrari is currently an Associate Research Professor in the Department of Surgery, Division of Surgical Sciences at Duke University Medical Center. He is directing the ELISPOT, CTL, and ADCC laboratories for the HVTN central laboratory at Duke. He has been serving as a WHO/UNAIDS Vaccine Initiative consultant for the past six years, and coordinated the organization of 5 international workshops for the transfer of ELISpot and ICS technologies to new laboratory sites involved in HIV vaccine studies.



Recent Publications

Chege, GK, Burgers, WA, Müller, TL, Gray, CM, Shephard, EG, Barnett, SW, Ferrari, G, Montefiori, D, Williamson, C, and Williamson, AL. "DNA-MVA-protein vaccination of rhesus macaques induces HIV-specific immunity in mucosal-associated lymph nodes and functional antibodies." Vaccine (January 6, 2017).

Luo, K, Liao, HX, Zhang, R, Easterhoff, D, Wiehe, K, Gurley, TC, Armand, LC, Allen, AA, Von Holle, TA, Marshall, DJ, Whitesides, JF, Pritchett, J, Foulger, A, Hernandez, G, Parks, R, Lloyd, KE, Stolarchuk, C, Sawant, S, Peel, J, Yates, NL, Dunford, E, Arora, S, Wang, A, Bowman, CM, Sutherland, LL, Scearce, RM, Xia, SM, Bonsignori, M, Pollara, J, Edwards, RW, Santra, S, Letvin, NL, Tartaglia, J, Francis, D, Sinangil, F, Lee, C, Kaewkungwal, J, Nitayaphan, S, Pitisuttithum, P, and Rerks-Ngarm, S et al. "Tissue memory B cell repertoire analysis after ALVAC/AIDSVAX B/E gp120 immunization of rhesus macaques." JCI insight 1, no. 20 (December 8, 2016): e88522-.

Huang, Y, Ferrari, G, Alter, G, Forthal, DN, Kappes, JC, Lewis, GK, Love, JC, Borate, B, Harris, L, Greene, K, Gao, H, Phan, TB, Landucci, G, Goods, BA, Dowell, KG, Cheng, HD, Bailey-Kellogg, C, Montefiori, DC, and Ackerman, ME. "Diversity of Antiviral IgG Effector Activities Observed in HIV-Infected and Vaccinated Subjects." Journal of immunology (Baltimore, Md. : 1950) 197, no. 12 (December 2016): 4603-4612.

Ferrari, G, Haynes, BF, Koenig, S, Nordstrom, JL, Margolis, DM, and Tomaras, GD. "Envelope-specific antibodies and antibody-derived molecules for treating and curing HIV infection." Nature reviews. Drug discovery 15, no. 12 (December 2016): 823-834.

Astronomo, RD, Santra, S, Ballweber-Fleming, L, Westerberg, KG, Mach, L, Hensley-McBain, T, Sutherland, L, Mildenberg, B, Morton, G, Yates, NL, Mize, GJ, Pollara, J, Hladik, F, Ochsenbauer, C, Denny, TN, Warrier, R, Rerks-Ngarm, S, Pitisuttithum, P, Nitayapan, S, Kaewkungwal, J, Ferrari, G, Shaw, GM, Xia, SM, Liao, HX, Montefiori, DC, Tomaras, GD, Haynes, BF, and McElrath, JM. "Neutralization Takes Precedence Over IgG or IgA Isotype-related Functions in Mucosal HIV-1 Antibody-mediated Protection." EBioMedicine 14 (December 2016): 97-111.

Nemes, E, Burgers, WA, Riou, C, Andersen-Nissen, E, Ferrari, G, Gray, CM, and Scriba, T. "Teaching advanced flow cytometry in Africa: 10 years of lessons learned." Cytometry. Part A : the journal of the International Society for Analytical Cytology 89, no. 11 (November 4, 2016): 971-974.

Costa, MR, Pollara, J, Edwards, RW, Seaman, MS, Gorny, MK, Montefiori, DC, Liao, HX, Ferrari, G, Lu, S, and Wang, S. "Fc Receptor-Mediated Activities of Env-Specific Human Monoclonal Antibodies Generated from Volunteers Receiving the DNA Prime-Protein Boost HIV Vaccine DP6-001." Journal of virology 90, no. 22 (November 2016): 10362-10378.

Gay, CL, Willis, SJ, Cope, AB, Kuruc, JD, McGee, KS, Sebastian, J, Crooks, AM, McKellar, MS, Margolis, DM, Fiscus, SA, Hicks, CB, Ferrari, G, and Eron, JJ. "Fixed-dose combination emtricitabine/tenofovir/efavirenz initiated during acute HIV infection; 96-week efficacy and durability." AIDS (London, England) 30, no. 18 (November 2016): 2815-2822.

Negri, D, Blasi, M, LaBranche, C, Parks, R, Balachandran, H, Lifton, M, Shen, X, Denny, T, Ferrari, G, Vescio, MF, Andersen, H, Montefiori, DC, Tomaras, GD, Liao, H-X, Santra, S, Haynes, BF, Klotman, ME, and Cara, A. "Immunization with an SIV-based IDLV Expressing HIV-1 Env 1086 Clade C Elicits Durable Humoral and Cellular Responses in Rhesus Macaques." MOLECULAR THERAPY 24, no. 11 (November 2016): 2021-2032.

Negri, D, Blasi, M, LaBranche, C, Parks, R, Balachandran, H, Lifton, M, Shen, X, Denny, T, Ferrari, G, Vescio, MF, Andersen, H, Montefiori, DC, Tomaras, GD, Liao, HX, Santra, S, Haynes, BF, Klotman, ME, and Cara, A. "Immunization with an SIV-based IDLV Expressing HIV-1 Env 1086 Clade C Elicits Durable Humoral and Cellular Responses in Rhesus Macaques." Molecular therapy : the journal of the American Society of Gene Therapy 24, no. 11 (November 2016): 2021-2032.

Gordon, SN, Liyanage, NP, Doster, MN, Vaccari, M, Vargas-Inchaustegui, DA, Pegu, P, Schifanella, L, Shen, X, Tomaras, GD, Rao, M, Billings, EA, Schwartz, J, Prado, I, Bobb, K, Zhang, W, Montefiori, DC, Foulds, KE, Ferrari, G, Robert-Guroff, M, Roederer, M, Phan, TB, Forthal, DN, Stablein, DM, Phogat, S, Venzon, DJ, Fouts, T, and Franchini, G. "Boosting of ALVAC-SIV Vaccine-Primed Macaques with the CD4-SIVgp120 Fusion Protein Elicits Antibodies to V2 Associated with a Decreased Risk of SIVmac251 Acquisition." Journal of immunology (Baltimore, Md. : 1950) 197, no. 7 (October 2016): 2726-2737.

Buchbinder, S, Grunenberg, N, Sanchez, B, Seaton, K, Ferrari, G, Moody, MA, Frahm, N, Montefiori, D, Goepfert, P, Baden, L, Hay, C, Yu, X, Gilbert, P, McElrath, MJ, Huang, Y, Robinson, H, Tomaras, G, and Grp, HVTN0S. "DNA/MVA HIV Vaccine Producing Virus-like Particles (VLPs) Is Well-tolerated and Induces Durable Functional Antibodies (Abs) in HVTN 094." October 2016.

Townsley, S, Mohamed, Z, Guo, W, McKenna, J, Cleveland, B, LaBranche, C, Beaumont, D, Shen, X, Yates, NL, Pinter, A, Tomaras, GD, Ferrari, G, Montefiori, DC, and Hu, SL. "Induction of Heterologous Tier 2 HIV-1-Neutralizing and Cross-Reactive V1/V2-Specific Antibodies in Rabbits by Prime-Boost Immunization." Journal of virology 90, no. 19 (October 2016): 8644-8660.

Shen, X, Basu, R, Kwa, S, Labranche, C, Beaumont, D, Ferrari, G, Wyatt, L, Moss, B, Montefiori, D, Tomaras, G, and Robinson, H. "MVA-expressed gp140 Boosts More Durable V1/V2 and gp120 Antibody Responses than gp120 Protein in Alum." October 2016.

Huang, Y, Tomaras, G, Andersen-Nissen, E, Dintwe, O, Morris, L, Grunenberg, N, Issacs, A, DiazGranados, C, Sinangil, F, Laher, F, Swann, E, DeRosa, S, Ferrari, G, Montefiori, D, Gilbert, P, McElrath, J, Corey, L, Gray, G, and HVTN097, . "The Relationship between Immune Responses to Tetanus Vaccine, Hepatitis B Vaccine and a Pox-protein HIV Vaccine Regimen." October 2016.

Zhang, R, Martinez, DR, Nguyen, QN, Pollara, J, Arifin, T, Stolarchuk, C, Foulger, A, Amos, JD, Parks, R, Himes, JE, Wang, M, Edwards, RW, Trama, AM, Vandergrift, N, Colvin, L, Dewar, K, Juretic, N, Wasserscheid, J, Ferrari, G, Liao, HX, and Permar, SR. "Envelope-specific B-cell populations in African green monkeys chronically infected with simian immunodeficiency virus." Nature communications 7 (July 6, 2016): 12131-.

Vaccari, M, Gordon, SN, Fourati, S, Schifanella, L, Liyanage, NP, Cameron, M, Keele, BF, Shen, X, Tomaras, GD, Billings, E, Rao, M, Chung, AW, Dowell, KG, Bailey-Kellogg, C, Brown, EP, Ackerman, ME, Vargas-Inchaustegui, DA, Whitney, S, Doster, MN, Binello, N, Pegu, P, Montefiori, DC, Foulds, K, Quinn, DS, Donaldson, M, Liang, F, Loré, K, Roederer, M, Koup, RA, McDermott, A, Ma, ZM, Miller, CJ, Phan, TB, Forthal, DN, Blackburn, M, Caccuri, F, Bissa, M, Ferrari, G, Kalyanaraman, V, and Ferrari, MG et al. "Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition." Nature medicine 22, no. 7 (July 2016): 762-770.

Rountree, W, Berrong, M, Sanchez, AM, Denny, TN, and Ferrari, G. "Variability of the IFN-%u03B3 ELISpot assay in the context of proficiency testing and bridging studies." Journal of immunological methods 433 (June 2016): 69-76.

Nelson, CS, Pollara, J, Kunz, EL, Jeffries, TL, Duffy, R, Beck, C, Stamper, L, Wang, M, Shen, X, Pickup, DJ, Staats, HF, Hudgens, MG, Kepler, TB, Montefiori, DC, Moody, MA, Tomaras, GD, Liao, HX, Haynes, BF, Ferrari, G, Fouda, GG, and Permar, SR. "Combined HIV-1 Envelope Systemic and Mucosal Immunization of Lactating Rhesus Monkeys Induces a Robust Immunoglobulin A Isotype B Cell Response in Breast Milk." Journal of virology 90, no. 10 (May 2016): 4951-4965.

Clutton, GT, Xu, Y, Fernandez, MA, Crooks, A, Turner, B, Maponga, N, Cope, A, Fiscus, S, McGee, K, McKellar, M, Kuruc, J, Ferrari, G, Margolis, D, Hicks, C, Eron, J, Gay, C, and Goonetilleke, N. "Effects of different treatment regimens on T cell function in acute HIV infection." May 1, 2016.

Asbach, B, Kliche, A, Köstler, J, Perdiguero, B, Esteban, M, Jacobs, BL, Montefiori, DC, LaBranche, CC, Yates, NL, Tomaras, GD, Ferrari, G, Foulds, KE, Roederer, M, Landucci, G, Forthal, DN, Seaman, MS, Hawkins, N, Self, SG, Sato, A, Gottardo, R, Phogat, S, Tartaglia, J, Barnett, SW, Burke, B, Cristillo, AD, Weiss, DE, Francis, J, Galmin, L, Ding, S, Heeney, JL, Pantaleo, G, and Wagner, R. "Potential To Streamline Heterologous DNA Prime and NYVAC/Protein Boost HIV Vaccine Regimens in Rhesus Macaques by Employing Improved Antigens." Journal of virology 90, no. 8 (April 2016): 4133-4149.

Jeffries, TL, Sacha, CR, Pollara, J, Himes, J, Jaeger, FH, Dennison, SM, McGuire, E, Kunz, E, Eudailey, JA, Trama, AM, LaBranche, C, Fouda, GG, Wiehe, K, Montefiori, DC, Haynes, BF, Liao, HX, Ferrari, G, Alam, SM, Moody, MA, and Permar, SR. "The function and affinity maturation of HIV-1 gp120-specific monoclonal antibodies derived from colostral B cells." Mucosal immunology 9, no. 2 (March 2016): 414-427.

Joachim, A, Bauer, A, Joseph, S, Geldmacher, C, Munseri, PJ, Aboud, S, Missanga, M, Mann, P, Wahren, B, Ferrari, G, Polonis, VR, Robb, ML, Weber, J, Tatoud, R, Maboko, L, Hoelscher, M, Lyamuya, EF, Biberfeld, G, Sandström, E, Kroidl, A, Bakari, M, Nilsson, C, and McCormack, S. "Boosting with Subtype C CN54rgp140 Protein Adjuvanted with Glucopyranosyl Lipid Adjuvant after Priming with HIV-DNA and HIV-MVA Is Safe and Enhances Immune Responses: A Phase I Trial." PloS one 11, no. 5 (January 2016): e0155702-.

Cosgrove, CA, Lacey, CJ, Cope, AV, Bartolf, A, Morris, G, Yan, C, Baden, S, Cole, T, Carter, D, Brodnicki, E, Shen, X, Joseph, S, DeRosa, SC, Peng, L, Yu, X, Ferrari, G, Seaman, M, Montefiori, DC, Frahm, N, Tomaras, GD, Stöhr, W, McCormack, S, and Shattock, RJ. "Comparative Immunogenicity of HIV-1 gp140 Vaccine Delivered by Parenteral, and Mucosal Routes in Female Volunteers; MUCOVAC2, A Randomized Two Centre Study." PloS one 11, no. 5 (January 2016): e0152038-.

Costa, MR, Pollara, J, Edwards, RW, Seaman, MS, Gorny, MK, Montefiori, DC, Liao, HX, Ferrari, G, Lu, S, and Wang, S. "Fc receptor-mediated activities of Envspecific human monoclonal antibodies generated from volunteers receiving the DNA prime-protein boost HIV vaccine DP6-001." Journal of Virology 90, no. 22 (January 1, 2016): 10362-10378.

Zurawski, G, Zurawski, S, Flamar, AL, Richert, L, Wagner, R, Tomaras, GD, Montefiori, DC, Roederer, M, Ferrari, G, Lacabaratz, C, Bonnabau, H, Klucar, P, Wang, Z, Foulds, KE, Kao, SF, Yates, NL, LaBranche, C, Jacobs, BL, Kibler, K, Asbach, B, Kliche, A, Salazar, A, Reed, S, Self, S, Gottardo, R, Galmin, L, Weiss, D, Cristillo, A, Thiebaut, R, Pantaleo, G, and Levy, Y. "Targeting HIV-1 Env gp140 to LOX-1 Elicits Immune Responses in Rhesus Macaques." PloS one 11, no. 4 (January 2016): e0153484-.